John Rumberger awarded Doctor of Philosophy by the University of Buckingham
24 June 2009
John Rumberger was an external student working at the Bassett Healthcare Research Institute, Cooperstown, New York State, USA. His local supervisor was Dr Allan Green and his University of Buckingham supervisor was Professor Jon Arch. His DPhil thesis is entitled ‘Nutritional regulation of lipolysis in adipocytes’.
Lipolytic studies in general have focused on the short-term regulation with relatively little understanding of the long-term regulation. However, the long-term regulation of lipolysis is a crucial element of metabolic diseases such as type 2 diabetes. John studied the long-term regulation by short-chain fatty acids and glucose. He found that short-chain fatty acids inhibit the enzyme histone deacetylase, which regulates transcriptional activity. By using inhibitors of different pathways of glucose metabolism and a gene silencing approach (shRNA), he showed that the metabolism of glucose via the glycolytic pathway regulates the expression of lipolytic genes.